PCR gene amplification instrument type introduction - Database & Sql Blog Articles

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PCR gene amplification instruments are designed based on the principles of DNA denaturation, renaturation, and extension. They incorporate constant temperature control, rapid heat transfer, and fast temperature cycling, combined with sensing technology, microelectronics, and automation. These instruments are developed using computer technologies to ensure precision and efficiency in DNA amplification processes.

Bayu

The working principles of various PCR instruments are primarily analyzed from the perspective of temperature regulation and thermal cycling. Below is a detailed explanation of different types of PCR amplifiers:

1. Water Bath PCR Amplification Instrument: This type of instrument typically consists of three separate water baths at different temperatures and a robotic arm. The sample tubes are immersed in these baths for denaturation, annealing, and extension. The timing and movement between the baths are controlled by a microcomputer and robotic system. It offers adjustable temperature settings, high accuracy, and uniform heating, making it suitable for use in grassroots laboratories. However, mechanical arms may be prone to failure, and water evaporation can occur in the baths (mineral oil can help prevent this). Additionally, some models use a single fixed sample chamber, where temperature changes are achieved through automated pumping or discharging systems, though such designs are less common.

2. Airflow PCR Amplification Instrument: This instrument is mainly composed of a convection oven, a hot air source, a cold air source, and a controller. The sample tubes are placed inside the oven, and the controller manages the flow of hot and cold air to regulate the temperature of the reaction tubes. This system is cost-effective, safe, and has no liquid components, making it ideal for environments where liquid-based systems might pose risks.

3. Metal Modular PCR Amplification Instrument: The core component of this instrument is a metal block made of aluminum or stainless steel, featuring multiple sample tube holes. It uses resistance heating, compressor cooling, semiconductor temperature modulation, or electronic temperature control to achieve precise and rapid temperature changes. These instruments are compact, offer fast temperature transitions, and provide accurate and uniform heating. They support large-scale amplification and can handle various sample sizes, including 0.5ml tubes and thin-wall tubes. They are widely used in research institutions and diagnostic centers due to their reliability and performance, although they tend to be more expensive.

Shanghai Bayu

Shanghai Bayu Industrial Co., Ltd. established an offline supply system for laboratory instruments and MRO industrial products in 2007. In 2009, the company launched an online procurement platform. As one of the earliest enterprises in Shanghai specializing in MRO industrial products and laboratory equipment, the company has strong technical capabilities, scientific management, quality assurance, and a solid after-sales service system. With a reputation for integrity and reliability, it continues to serve customers across various industries.